Open AccessChemical constituents of the stem bark ofVochysia thyrsoideaPohl. (Vochysiaceae) and evaluation of their cytotoxicity and inhibitory activity against cathepsins B and K.
Author(s) -
Lorena Ramos Freitas de Sousa,
James A. da Silva,
Paulo Cézar Vieira,
Maísa Borges Costa,
Mirley Luciene dos Santos,
Aline Borba Sbardelotto,
Cláudia Pessoa,
Manoel Odoríco de Moraes,
Antônio Carlos Severo Menezes
Publication year - 2014
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.5935/0100-4042.20140049
Subject(s) - ic50 , inhibitory postsynaptic potential , catechin , cytotoxicity , chemistry , stereochemistry , biochemistry , bark (sound) , polyphenol , microbiology and biotechnology , biology , in vitro , endocrinology , ecology , antioxidant
A new flavonoid, catechin-3-O-(3"-O-trans-cinnamoyl)-α-rhamnopyranoside, along with known compounds, catechin-3-O-α-rhamnopyranoside, 3-oxo-urs-12-en-28-oic acid, 2,4,6-trimethoxybenzoic acid, 2-butyl-D-fructofuranoside and 1-butyl-D-fructofuranoside, has been isolated from the stem bark of V. thyrsoidea. These compounds were assayed for inhibition of protease activity (cathepsins B and K) and against cancer cell lines. Catechin-3-O-(3"-O-trans-cinnamoyl)-α-rhamnopyranoside showed moderate inhibitory activity (IC50 = 62.02 µM) against cathepsin B while 2-butyl-D-fructofuranoside was the most potent against a strain of CNS (SF-295) and human leukemia (HL-60) with IC50 = 36.80 µM and IC50 = 25.37 µM, respectively