Open AccessModulação do estado de polimerização in vitro dos filamentos intermediários em astrócitos pela fosforilação e proteína S100B
Author(s) -
Erica Bortoli,
Juliana Karl Frizzo,
Carlos Alberto Gonçalves
Publication year - 2003
Publication title -
ciência e natura
Language(s) - English
Resource type - Journals
eISSN - 2179-460X
pISSN - 0100-8307
DOI - 10.5902/2179460x27235
Subject(s) - glial fibrillary acidic protein , chemistry , in vitro , cytoskeleton , polymerization , depolymerization , microbiology and biotechnology , protein kinase c , calcium , phosphorylation , biophysics , biochemistry , biology , cell , polymer chemistry , immunology , polymer , immunohistochemistry , organic chemistry
Intermediate filaments represent a major cytoskeleton constituent in eukaryotic cells. GFAP ("glial fibrillary acidic protein") is the monomer of these filaments in astrocytes and its polymerization is apparently modulated by phosphorylation and by interaction with S100B, a calcium-binding protein. In this study we investigate in vitro polymerization of GFAP, using an assay based on imidazol/high magnesium-induced sedimentation. In fact, soluble GFAP (nonpolymerized) increased about 25% in presence of S100B or PKA (protein kinase A). These data suggest that both mechanisms could be acting in the cycle of polymerization/depolymerization of GFAP at different times and/or conditions, therefore affecting glial plasticity.