Open AccessMicrodroplet Protein Sensors on a Gold Surface with a Self-assembled Monolayer Treatment
Author(s) -
Tzu-Chun Liao,
J. Andrew Yeh
Publication year - 2012
Publication title -
international journal of automation and smart technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.148
H-Index - 10
ISSN - 2223-9766
DOI - 10.5875/ausmt.v2i1.116
Subject(s) - monolayer , bovine serum albumin , surface tension , materials science , contact angle , silicon , layer (electronics) , self assembled monolayer , surface modification , nanotechnology , analytical chemistry (journal) , chemistry , chromatography , composite material , optoelectronics , physics , quantum mechanics
A new kind of microdroplet-based biological protein sensor is presented. The sensor was made by placing silicon oil on gold film with a self-assembled monolayer (SAM). The surface tension dominates the sensitivity of the sensor. Using mercaptoundecanoic acid (11-MUA) as the sensor’s SAM layer, the sensor can detect 0.5 mg/ml, 20 μg/ml, and 0.4 μg/ml bovine serum albumin (BSA) protein solutions in a control volume of 0.5ml. The sensor’s reaction time for concentrations of 0.5 mg/ml, 20 μg/ml, and 0.4μg/ml protein solutions was 15, 60 and 120 minutes, respectively. As the size of microdroplet decreased, the change of contact angle increased