Open AccessIn Vitro Oocyte Maturation Rate of Mice with Polycystic Ovarian Syndrome in the Presence of α-Linolenic Acid Antioxidant
Author(s) -
Amaneh Moradi,
Fatemeh Ghasemian,
Farhad Mashayekhi
Publication year - 2020
Publication title -
majallah-i taḥqīqāt-i ̒ulūm-i pizishkī-i zāhidān/majallah-i taḥqīqāt-i ̒ulūm-i pizishkī-i zāhidān./zahedan journal of researches in medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2228-6403
pISSN - 2008-7977
DOI - 10.5812/zjrms.95100
Subject(s) - tfam , polycystic ovary , in vitro maturation , oocyte , estradiol valerate , folliculogenesis , endocrinology , andrology , medicine , biology , bone morphogenetic protein 15 , germinal vesicle , oogenesis , estrogen , mitochondrial biogenesis , embryo , mitochondrion , gene , embryogenesis , insulin resistance , obesity , biochemistry , bone morphogenetic protein , microbiology and biotechnology , bone morphogenetic protein 7
Background: One of the most common endocrine and metabolic disorders is Polycystic ovary syndrome (PCOS), which has been reported in about 10% of women during the reproductive age. Objectives: This study was designed to investigate the efficiency of α-Linolenic acid (ALA) on in vitro maturation (IVM) and the quality of mouse oocytes with PCOS. Methods: Female NMRI mice (30 - 35 day-old) were developed by the injection of 4 mg estradiol valerate dissolved in 0.2 mg sesame oil for 60 consecutive days. In the following, the PCOS ovaries were dissected and oocytes were cultured in the maturation medium supplemented with different dosages of α-linolenic acid (0, 50, 100 µM). The presence of the first polar body was considered the sign of the nuclear maturation of the oocyte. The expression of mitochondrial transcription factor A (TFAM) gene in mature oocytes was investigated by Quantitative Real-time PCR. Results: The in vitro maturation and TFAM gene expression rates of PCOS oocytes in the medium treated with 50 µM of ALA (84 ± 7.9 and 0.46 ± 0.09, respectively) were significantly higher than the control group (P < 0.05). Conclusions: The ALA could improve the IVM rate and quality of PCOS oocytes by higher expression of TFAM gene.