Open AccessComparison of Various Methods for Isolation of Nocardia from Soil
Author(s) -
Masoumeh Rasoulinasab,
Mehdi Fatahi-Bafghi,
Shadi Habibnia,
Parvin Heidarieh,
Seyyed Saeed Eshraghi
Publication year - 2017
Publication title -
majallah-i taḥqīqāt-i ̒ulūm-i pizishkī-i zāhidān/majallah-i taḥqīqāt-i ̒ulūm-i pizishkī-i zāhidān./zahedan journal of researches in medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2228-6403
pISSN - 2008-7977
DOI - 10.5812/zjrms.6107
Subject(s) - nocardia , agar , isolation (microbiology) , microbiology and biotechnology , biology , agar plate , chromatography , bacteria , chemistry , genetics
BackgroundThe genus Nocardia is Gram-positive, aerobic filamentous bacilli and saprophytic micro-organisms that can be isolated from freshwater, salt water, dust and decaying vegetation especially the soil. This study aimed to investigate the several media for to determine a suitable culture media with the ability to better for the isolation of Nocardia from soil.MethodsIn this study, 400 soil samples were collected from different areas from Iran. The soil samples were then cultured on the four culture media such as Humic acid vitamin B agar, Paraffin agar, Sabouraud dextrose agar supplemented whit cycloheximide and carbon-free broth containing paraffin rods and incubated at 35°C. All of culture media investigated every 3 days for a month. Colonies suspicious to Nocardia were stained with Gram-stain, acid-fast and partially acid-fast and evaluated for resistance to lysozyme.ResultsFrom 400 soil samples, the number of 62, 10, 28 and 19 strains of Nocardia were isolated by paraffin rods, Humic Acid Vitamin B agar, Paraffin agar and Sabouraud dextrose agar whit cycloheximide, respectively. Most Nocardia strains were isolated using paraffin bait technique.ConclusionsIsolation of Nocardia spp. is enhanced by using the paraffin baiting technique that relies on the selective ability of this micro-organism to metabolize paraffin