Open AccessNon Detection of HIV-1 Proviral DNA in PBMCs of the Neonates Born to Iranian HIV-Infected Mothers in PMTCT Program
Author(s) -
Zahra Habib,
Farah BokharaeiSalim,
Seyed Jalal Kiani,
Saba Garshasbi,
Saeed Kalantari,
Khadijeh Khanaliha,
Sedigheh Taghinezhad-S,
Seyed Hamidreza Monavari,
Angila Ataei Pirkooh,
Maryam Esghaei
Publication year - 2021
Publication title -
archives of pediatric infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 12
eISSN - 2322-1836
pISSN - 2322-1828
DOI - 10.5812/pedinfect.105098
Subject(s) - medicine , peripheral blood mononuclear cell , virology , transmission (telecommunications) , human immunodeficiency virus (hiv) , long terminal repeat , viral load , immunology , virus , genomic dna , pediatrics , dna , biology , gene , genome , genetics , in vitro , electrical engineering , engineering
Background: Early diagnosis of immunodeficiency virus-1 infection in children and access to treatment for this infection is critical in decreasing infant mortality. Objectives: The aim of the current survey was to determine the presence of HIV-1 genomic RNA in plasma and proviral DNA in peripheral blood mononuclear cell (PBMC) specimens of neonates born to HIV-infected mothers. Methods: From March 2014 to February 2018, 73 neonates born to HIV-1-infected mothers covered by the prevention of mother-to-child transmission (PMTCT) program were enrolled in this study to compare two different diagnostic methods. After the extraction of viral RNA of plasma and genomic DNA of PBMC specimens, HIV-1 RNA and proviral DNA was tested by amplification of the long terminal repeat (LTR) region of HIV-1 using real-time PCR. Results: Out of 73 evaluated infants, 41 infants (56.2%) were male. The average age of the mothers with HIV-1 infection was 30.7 ± 5.2 (range: 19–47) years. The results revealed that none of the infants were infected with HIV-1, and also all were negative for HIV-1 genomic RNA in plasma specimen and proviral DNA of HIV-1 in PMBC samples. During the present study, 20 infants born to HIV-1 positive mothers who were not included in the PMTCT project were accidentally identified. Four infants (20%) out of these 20 infants were infected with HIV, all were infected with CRF35-AD of HIV, and none carried variants with surveillance drug-resistant mutations. Conclusions: The results of the present study showed that two molecular methods of detecting HIV infection (presence of genomic RNA of HIV-1 in plasma and proviral DNA of HIV-1 in PBMC specimens) are completely in agreement with each other, and the PMTCT program is possibly an effective program.