Open AccessThe Regulation of Testosterone Levels and Improvement of Sperm DNA Maturity and In-Vitro Fertilization Outcome by Selenium Administration in Diazinon-treated Wistar Rats
Author(s) -
Ghodrat Ebadimanas,
Gholamreza Najafi
Publication year - 2020
Publication title -
journal of kermanshah university of medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2588-2570
pISSN - 2588-2562
DOI - 10.5812/jkums.103154
Subject(s) - diazinon , sperm , human fertilization , andrology , selenium , sperm motility , testosterone (patch) , in vitro fertilisation , biology , medicine , embryo , endocrinology , chemistry , anatomy , genetics , pesticide , ecology , organic chemistry
Background: Diazinon is an insecticide with oxidative stress properties, which is widely used in agriculture. Selenium is used as a basic nutrient for animals and humans as a potential antioxidant. No studies have evaluated the protective effects of selenium on diazinon in the reproductive system of male Wistar rats. Objectives: The present study aimed to assess the therapeutic effects of selenium on balanced testosterone levels, sperm DNA damage, fertilization, and early embryonic development in diazinon-treated rats. Methods: This experimental study was conducted on 32 mature male Wistar rats, which were randomly assigned to four groups of eight, including control (0.2 mg/kg/B.W of corn oil), diazinon (300 mg/kg/B.W), selenium (0.5 mg of Na2SeO3/kg), selenium (0.5 mg/kg), and diazinon (300 mg/kg/B.W of diazinon). The animals received daily oral treatment for 60 days. Results: Compared to the control group, the diazinon group showed a significant reduction in the testosterone levels, sperm count, sperm motility, sperm maturation, DNA maturity, in-vitro fertilization (IVF), two-cell embryo, hatching, and blastocyst (P value < 0.05). However, the sperm mortality, sperm DNA damage, and immature sperm significantly increased in the treatment group (P value < 0.05). In the diazinon and selenium treatment group, the total sperm parameters returned to the physiological range, and no significant changes were observed. Conclusions: According to the results, selenium could protect the sperm parameters, testosterone level, and IVF in the diazinon-treated Wistar rats.