Open AccessMelatonin Effect on Immature Mouse Testicular Tissues, Vitrified-Thawed With Different Cryoprotectant Media
Author(s) -
Mohammadreza Gholami,
Ghasem Saki,
Masoud Hemadi,
Ali Khodadadi,
Javad Mohammadi-Asl
Publication year - 2015
Publication title -
jentashapir journal of health research
Language(s) - English
Resource type - Journals
ISSN - 2345-4067
DOI - 10.5812/jjhr.28704v2
Subject(s) - melatonin , cryoprotectant , andrology , haematoxylin , cryopreservation , eosin , glycerol , antioxidant , vitrification , medicine , h&e stain , staining , endocrinology , biology , embryo , pathology , biochemistry , microbiology and biotechnology
BackgroundFreezing of the immature testis tissue and then its transplantation can be considered a major step in fertility preservation for young boys with cancer.ObjectivesThe objective of this study was to investigate the antioxidant effects of melatonin on histological structure of seminiferous tubules that were vitrified-thawed with different cryoprotectant media.Materials and MethodsMale mice were randomly assigned to one of 10 experimental groups. Testes of 6-day-old BALB/c male mice pups were extracted and vitrified-thawed with different vitrification media with melatonin and without melatonin. Then, their testes were stained with haematoxylin-eosin.ResultsThe result showed that supplementation of glycerol based media with melatonin has reduced injury to histological structure of seminiferous tubules in the 6-day-old male BALB/c mice pups testes (P = 0.004).ConclusionsIt seem that melatonin due to its antioxidant properties, if added to glycerol based media, reduces the harmful effects of freezing-thawing process, and protects seminiferous tubules histological structure from oxidative injury