Open AccessHigh Expression of TWIST-1 in the Chronic Myeloid Leukemia Patients with T315I Mutation
Author(s) -
Nazanin Heidari,
Fatemeh Noroozi,
Najmaldin Saki
Publication year - 2021
Publication title -
jentashapir journal of cellular and molecular biology
Language(s) - English
Resource type - Journals
ISSN - 2717-2724
DOI - 10.5812/jjcmb.118718
Subject(s) - myeloid leukemia , mutation , medicine , polymerase chain reaction , gene , gene mutation , gene expression , biology , cancer research , genetics
Background: Among the known ABL mutations in chronic myeloid leukemia (CML), T315I is of particular importance. The T315I mutation may develop resistant cells that increase disease progression. TWIST-1 expression is impaired in patients with increased drug resistance. Objectives: The current study aimed to measure the expression of TWIST-1 gene in CML patients to investigate its association with T315I mutation. Methods: Peripheral blood samples were taken from 40 CML patients. The expression of TWIST-1 and BCR-ABL1 genes was quantified by real-time polymerase chain reaction (PCR). The gene expression was evaluated by REST software. cDNA was used for amplification refractory mutation system (ARMS)-PCR reaction. Results: Of the 40 patients (age range: 19 - 72 years) participating in the study, 23 (57.7%) were female, and 17 (42.5%) were male. The expression of TWIST-1 gene was 43 ± 184.09-fold. The T315I mutation was detected in 3 (7.5%) patients. Conclusions: According to our results, the TWIST-1 gene expression in patients with T315I mutation was significantly higher than patients without that mutation.