Open AccessAntioxidant Property and Bactericidal Activity of Linum usitatissimum Seed Essential Oil Nanoemulsion (LSEO-NE) on Staphylococcus aureus
Author(s) -
Roghaye Keykhasalar,
Masoud Homayouni Tabrizi,
Pouran Ardalan
Publication year - 2020
Publication title -
international journal of infection
Language(s) - English
Resource type - Journals
eISSN - 2383-1421
pISSN - 2383-1413
DOI - 10.5812/iji.101639
Subject(s) - staphylococcus aureus , dpph , linum , antioxidant , antibacterial activity , abts , chemistry , antimicrobial , microbiology and biotechnology , kanamycin , essential oil , food science , traditional medicine , biochemistry , antibiotics , biology , bacteria , botany , medicine , genetics
Background: Linum usitatissimum Seed Essential Oil (LSEO) as an efficient antimicrobial compound contains various types of phytochemicals, such as lignans and phenols. Objectives: In the current study, we produced LSEO nanoemulsion (LSEO-NE) to study its antioxidant capacity and bactericidal activity against Staphylococcus aureus. Methods: The LSEO-NE was produced using the ultrasonication method and characterized by Atomic Force Microscopy (AFM), Dynamic Light Scattering (DLS), and Scanning Electron Microscopy (SEM). Then, we measured its antioxidant activity utilizing both ABTS and DPPH tests at four different LSEO-NE concentrations (200, 600, 800, and 1000 µg/mL) compared to glutathione. Finally, we evaluated its bactericidal activity on S. aureus by studying Antibiotic Susceptibility Testing (AST) using LSEO-NE-smeared discs compared to non-smeared and kanamycin discs. Results: The 67.3 nm droplets of LSEO-NE with PDI of 0.452 exhibited strong antioxidant activity, similar to glutathione, in both ABTS (IC50 = 350 µg/mL) and DPPH (IC50 = 235 µg/mL) tests. Moreover, the AST results revealed the significant sensitivity of S. aureus to LSEO-NE-smeared discs when compared to non-smeared and kanamycin discs. Conclusions: According to the results, LSEO-NE can be applied as a safe, natural, and effective antibiotic for bacterial infections caused by S. aureus in most organs, such as the respiratory system and skin. However, further in vivo studies are required to evaluate the LSEO-NE antibacterial efficiency against other pathogenic S. aureus strains.