Open AccessMicroRNA Prediction in the FVIII Gene Locus: A Step Towards Hemophilia A Control
Author(s) -
Hadi Rezaei,
Majid Motovali-Bashi,
Sheyda Khalilian
Publication year - 2020
Publication title -
gene, cell and tissue
Language(s) - English
Resource type - Journals
eISSN - 2345-6841
pISSN - 2345-6833
DOI - 10.5812/gct.103096
Subject(s) - mirbase , microrna , locus (genetics) , gene , computational biology , disease , biology , biomarker , biomarker discovery , bioinformatics , genetics , medicine , proteomics , pathology
Background: Various mutations in factor VIII (F8) gene locus are led to an X-linked bleeding disorder in patients with hemophilia A. One of the leading causes of inefficient treatment available for hemophilia A is the lack of specific and sensitive diagnostic procedure for the disease. The discovery of a functional role of microRNAs (miRNAs) in the pathogenesis of a wide range of human diseases makes them the potential, non-invasive, biomarker candidates for hemophilia A. Therefore, advances in computational tools for miRNA discovery leads to numerous recent publications on miRNAs as putative biomarkers. Objectives: The current study aimed at scanning the F8 gene region to predict novel miRNAs as regulators of the F8 gene. Methods: The potential of the FVIII locus to express new miRNAs was studied via reliable bioinformatics databases, such as SSCprofiler, RNAfold, miREval, miR-Find, FOMmiR, UCSC genome browser, and miRBase. Results: Data analysis from previously mentioned databases offered two stem-loop structures predicted to express novel miRNAs. Conclusions: The presented stem-loop structures can be used as powerful non-invasive biomarkers in early diagnosis of the disease and regulation of the factor VIII gene after subsequent experimental verification.