Open AccessMolecular characterization and antibiogram of Listeria monocytogenes isolated from chicken and mutton of retail markets
Author(s) -
Vinay Kumar B. N
Publication year - 2016
Publication title -
journal of microbiology and infectious diseases
Language(s) - English
Resource type - Journals
eISSN - 2146-9369
pISSN - 2146-3158
DOI - 10.5799/ahinjs.02.2016.02.0218
Subject(s) - listeria monocytogenes , virulence , antibiogram , biology , hemolysin , microbiology and biotechnology , food science , listeria , cooked meat , poultry meat , food safety , gene , bacteria , antibiotic resistance , antibiotics , genetics , biochemistry
Objective: To isolate Listeria monocytogenes from chicken and mutton meat sold at retail outlets and to characterize\udisolates for virulence determinants.\udMethods: Listeria monocytogenes was isolated from chicken and mutton meat samples using ISO: 11290 method. Multiplex\udPCR was performed for the detection of virulence associated genes.\udResults: Out of 198 retail meat samples (72 chicken and 126 mutton) analyzed, Listeria monocytogenes was isolated\udfrom 4.5% (8.3% chicken and 2.3% mutton) samples. All the 9 isolates (6 from chicken and 3 from mutton) belonged to\ud1/2a servar and carried virulence genes viz. haemolysin (hlyA), phosphatidylinositol phospholipase (plcA), actin polymerization\udprotein (actA) and invasive associative protein p60 (Iap).\udConclusion: L. monocytogenes is an organism of food safety and public health significance, its recovery from the meat\udsold at retail outlets indicated breach in the quality assurance. J Microbiol Infect Dis 2016;6(2): 65-6