Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate | Zendy

Chao Meng | Zendy; Zhao Dai | Zendy; Wenjuan Guo | Zendy; Yuanyuan Chu | Zendy; Guangping Chen | Zendy

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Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Author(s) -

Chao Meng,

Zhao Dai,

Wenjuan Guo,

Yuanyuan Chu,

Guangping Chen

Publication year - 2016

Publication title -

nanomaterials and nanotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.412

H-Index - 21

ISSN - 1847-9804

DOI - 10.5772/63985

Subject(s) - aptamer , förster resonance energy transfer , biosensor , adenosine triphosphate , colloidal gold , detection limit , fluorescence , dna , materials science , acceptor , nanotechnology , nanoparticle , analytical chemistry (journal) , biophysics , chemistry , chromatography , microbiology and biotechnology , biochemistry , biology , physics , quantum mechanics , condensed matter physics

A fluorescence resonance energy transfer (FRET)- based aptamer biosensor (aptasensor) for sensitive determination of adenosine triphosphate (ATP) was developed. The energy donor and acceptor were 5′-carboxyfluorescein (5′- FAM) and gold nanoparticles (AuNPs), respectively. AuNPs were linked to 3′-SH-DNA by Au-S bond. This aptasensor was obtained by the hybridization between the complementary DNA strands of the 5′-FAM and AuNPs. The detecting results indicated that the FRET increase efficiency (Fr) of detecting system at 519 nm was linear when the concentration of ATP target was about 0.1–10 mmol/L. Moreover, the aptasensors showed a good stability in different buffer conditions and a low- detection limit (15.2 nmol/L) for ATP

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