Open AccessSynthesis of Silver Nanoparticles by a Bryophilous Rhizoctonia Species
Author(s) -
Daniel B. Raudabaugh,
Marian Tzolov,
Joseph Calabrese,
Barrie E. Overton
Publication year - 2013
Publication title -
nanomaterials and nanotechnology.
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.412
H-Index - 21
ISSN - 1847-9804
DOI - 10.5772/56207
Subject(s) - nitrate reductase , silver nanoparticle , hypha , nanoparticle , silver nitrate , surface plasmon resonance , spore , materials science , nitrate , nanotechnology , chemical engineering , microbiology and biotechnology , nuclear chemistry , chemistry , biology , organic chemistry , engineering
We demonstrate the synthesis of silver nanoparticlesby a potentially benign species of bryophilousRhizoctonia in two different media. The first mediumsupports fungal growth and the up‐regulation of nitratereductase, while the second medium supports fungalgrowth and the repression of nitrate reductase. For bothmedia, the resulting silver nanoparticles were ca. 25‐50nm and were subglobose to broadly ellipsoidal inshape. The optical analysis of the silver nanoparticlesfrom both media demonstrated plasmon resonance at415nm, confirming their metallic properties. The liquidcolour change typically observed for extracellular silvernanoparticle formation was absent in both media. Thesilver nanoparticles in the two different media displayeddifferent chemical associations; fewer associatedchemicals were found with the media, which supportedthe up‐regulation of nitrate reductase. Another differenceincluded plate‐like silver nanoparticle conglomerations,which were only encountered on hyphae from themedium that repressed nitrate reductase. There was alsoa noticeable difference in the capping agent formationsbetween each media. The Rhizoctonia isolate examined inthis study is suitable for large‐scale industrialapplications because it does not produce spores andwould have minimal impact on air quality