Open AccessEFEITO DE MEIOS DILUENTES E SISTEMAS DE TRANSPORTE NO SÊMEN CONGELADO ANALISADO PELO MÉTODO COMPUTACIONAL CASA EM TOUROS NELORE
Author(s) -
Talita Raquel Cavichioli Sebastião,
Camila Dutra de Souza Francisquini,
Marcelo George Mungai Chacur
Publication year - 2018
Publication title -
colloquium agrariae
Language(s) - English
Resource type - Journals
ISSN - 1809-8215
DOI - 10.5747/ca.2018.v14.n4.a257
Subject(s) - semen , zoology , andrology , biology , cryopreservation , semen quality , chemistry , medicine , embryo , microbiology and biotechnology
The objective was to evaluate diluents and transport systems on semen quality frozen by CASA in Nellore bulls. Five semen collections of six bulls, diluted with the TRISand BotuBOV®, were transported out on two types of BotuBOX® and BotuFLEX® systems; frozen and analyzed by CASA. TM was higher (p <0.05) in the association of BotuBOV® + BotuFLEX® (47.3%) when compared to TRIS + BotuBOX® (9%). The PM was higher (p <0.05) BotuBOV® + BotuFLEX® (37%) when compared to TRIS + BotuBOX® (5.9%). The same for VSL, BCF and RAP, which were higher (p <0.05) in BotuBOV® + BotuFLEX® (65.1μm/s, 30Hz, 44.5%, respectively) when compared to semen in the TRIS + BotuBOX® (47.6μm/s, 21.5Hz, 6.9%, respectively). It is concluded that BotuBOV® in association with the refrigerated system of semen BotuFLEX® was considered by CASA to be the best association when referring to sperm kinetics for cryopreservation of bovine semen.