EMBRIOGÊNESE SOMÁTICA DIRETA EM FOLHAS DE PHALAENOPSIS: ORCHIDACEASE

Phalaenopsis orchids are a largely produced floriculture genus due to their large, flat, highly variable in coloured and durable flowers arranged in long branches. They are fast growing plants and highly valuable. One of the propagation ways is the tissue culture. However, even with the finest propagation techniques of clone propagation may produce calluses and consequently may lead to somaclonal variation, an undesirable side effect. To minimize calluses formation a protocol of embryogenesis was tested in leaves of commercial plants. A series of combination between NAA and BAP was tested. The number of dead, oxidized, surviving and embryos per explant was analyzed. The combinations of 0.1mg L-1 of or 0.2mg L-1 of NAA and 1mg L-1BAP was the most effective to induce embryo formation