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Culture shock
Author(s) -
Gilbert Gwendolyn L,
James Gregory S,
Sintchenko Vitali
Publication year - 1999
Publication title -
medical journal of australia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 131
eISSN - 1326-5377
pISSN - 0025-729X
DOI - 10.5694/j.1326-5377.1999.tb123789.x
Subject(s) - nucleic acid , multiplex polymerase chain reaction , polymerase chain reaction , virulence , biology , multiplex , computational biology , dna , gene , microbiology and biotechnology , genetics
Nucleic acid detection methods, such as polymerase chain reaction (PCR), can often detect specific microbial pathogens, virulence markers and antimicrobial resistance genes more rapidly and with greater sensitivity and specificity than culture and conventional identification and susceptibility testing. Multiplex PCR can detect multiple genes in a single assay; this capability will be greatly extended by new techniques such as the DNA chip. However, limitations and pitfalls of nucleic detection methods remain.