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Efficient primary isolation of human immunodeficiency virus
Author(s) -
Pietroboni Glenys R.,
Harnett Gerald B.,
Bucens Marion R.
Publication year - 1989
Publication title -
medical journal of australia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 131
eISSN - 1326-5377
pISSN - 0025-729X
DOI - 10.5694/j.1326-5377.1989.tb136528.x
Subject(s) - virology , human immunodeficiency virus (hiv) , isolation (microbiology) , virus isolation , medicine , virus , biology , microbiology and biotechnology
A highly‐sensitive and efficient culture technique for human immunodeficiency virus type‐1 (HIV‐1) is described; HIV‐1 was recovered from the lymphocytes of 44 (94%) antibody‐seropositive healthy or symptomatic individuals. The reductions in the requirements for both the reagent volume and the number of patients' lymphocytes, together with an increased efficiency, has made this HIV‐1 culture system more practical for diagnostic virology laboratories. (Med J Aust 1989; 150: 376‐377)