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CYTOGENETIC STUDIES IN CHRONIC LYMPHOCYTIC LEUKÆMIA 2. STATISTICAL STUDIES OF G–GROUP CHROMOSOMES
Author(s) -
Woodliff H. J.,
Leong A.,
Stennouse N. S.
Publication year - 1972
Publication title -
medical journal of australia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 131
eISSN - 1326-5377
pISSN - 0025-729X
DOI - 10.5694/j.1326-5377.1972.tb108066.x
Subject(s) - karyotype , chronic lymphocytic leukemia , chromosome , biology , abnormality , cytogenetics , aneuploidy , pathology , genetics , medicine , leukemia , psychiatry , gene
Most cytogenetic studies of leukæmic lymphocytes from patients with chronic lymphocytic leukæmia have revealed no abnormality. However, in some, an increased amount of aneuploidy has been detected, and less commonly, pseudodiploidy has been described. Fitzgerald (1965 a, b) found that the total length of the G‐group chromosomes was shorter in chronic lymphocytic leukæmia when compared with controls. He thought that this might be due to the greater degree of spiralization and condensation of one or more of the small acrocentric chromosomes. We have studied this further by measuring all the lengths and areas of the G‐group chromosomes from patients with chronic lymphocytic leukæmia. A preliminary study of different methods of area measurements defined the coefficient of variance and planimetry was chosen as the method of measurement. A second preliminary study tested the experimental error by the analysis of duplicates and indicated that there was no great difference between length and area measurements. The definitive study was made on five male and five female patients, and on the same number of controls. The ratios of the lengths and the areas of the A1 to the G (or the G + Y in the males) chromosomes were measured in 10 metaphases from each subject. No significant differences were found between the ceils from leukæmic and normal subjects.