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IN‐VIVO EXPERIMENTS WITH ETHYLENEDIAMINE‐TETRA‐ACETIC ACID AND INVESTIGATIONS INTO ITS ACTION ON PENICILLIN‐RESISTANT STAPHYLOCOCCUS AUREUS
Author(s) -
Beaumont Rosemary J.
Publication year - 1970
Publication title -
medical journal of australia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 131
eISSN - 1326-5377
pISSN - 0025-729X
DOI - 10.5694/j.1326-5377.1970.tb63322.x
Subject(s) - in vivo , staphylococcus aureus , calcium , chemistry , chelation , penicillin , microbiology and biotechnology , enzyme , in vitro , magnesium , biochemistry , acetic acid , nuclear chemistry , antibiotics , bacteria , biology , inorganic chemistry , organic chemistry , genetics
EDTA was tested in vitro against penicillin‐resistant Staphylococcus aureus, together with penicillin at concentrations normally achieved in patients' serum. The results showed EDTA to be of no clinical use in this field. in‐vivo experiments, using infected mice as subjects, confirmed these findings. By use of a synthetic growth medium lacking calcium and magnesium ions, penicillinase was demonstrated to be uninhibited by EDTA, but the production of this enzyme was inhibited. Calcium and magnesium ions were shown to be necessary for penicillinase production, and EDTA chelates these ions, inhibiting the enzyme production. The penicillinase gene and plasmid were unaffected by the action of EDTA.