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TITRES, ACTIVITY OR SURVIVAL? THE QUANTITATION OF ANTI–Rh (D) GAMMA GLOBULIN
Author(s) -
Simmons K. T.
Publication year - 1969
Publication title -
medical journal of australia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 131
eISSN - 1326-5377
pISSN - 0025-729X
DOI - 10.5694/j.1326-5377.1969.tb107497.x
Subject(s) - gamma globulin , globulin , microgram , titration , chemistry , albumin , chromatography , antibody , medicine , immunology , biochemistry , in vitro , inorganic chemistry
The various stimuli leading up to the large‐scale preparation of anti‐Rh(D) gamma globulin in Australia early in 1967 and subsequently are mentioned. The quantitation of anti‐Rh(D) gamma globulin, namely, “titres”, by laboratory serum‐albumin titration methods, “activity”, by microgram‐permillilitre anti‐D estimations made in London and in Melbourne, and “survival”, by radioactivity estimations in Sydney of clearance times of 51 Cr‐tagged red cells conducted in Rh‐negative volunteers, and the necessary quality control tests at various stages of the preparation of gamma globulin are discussed.