Open AccessFicus elastica Leaf Extract Effect Toward IL-10 and TNF-α as a Preeclampsia Model on Hypoxia-induced EA.hy926
Author(s) -
Chrismis Novalinda Ginting,
I Nyoman Ehrich Lister,
Ermi Girsang,
Maya Sari Mutia,
Yolanda Eliza Putri Lubis,
Annisa Amalia,
Wahyu Setia Widodo,
Dwi Surya Artie,
Rizal Rizal,
Wahyu Widowati
Publication year - 2022
Publication title -
journal of mathematical and fundamental sciences/journal of mathematical and fundamental siences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.216
H-Index - 12
eISSN - 2337-5760
pISSN - 2338-5510
DOI - 10.5614/j.math.fund.sci.2021.53.3.10
Subject(s) - preeclampsia , hypoxia (environmental) , cytotoxicity , medicine , tumor necrosis factor alpha , traditional medicine , cytotoxic t cell , pregnancy , proteinuria , andrology , immunology , endocrinology , pharmacology , biology , chemistry , in vitro , biochemistry , genetics , organic chemistry , oxygen , kidney
Preeclampsia is a pregnancy complication characterized by proteinuria, hypertension, hypoxia as well an increased systemic inflammatory response. It affects 5 to 10% of all births and is a leading factor in fetal and maternal morbidity and death. Ficus elastica L. leaves are known to have natural compounds with anti-inflammatory and antioxidant properties. This research assessed the potential of F. elastica leaf extract (FEE) as anti-inflammatory agent in a hypoxic-induced human endothelial cell line (EA.hy926) as preeclampsia model. F. elastica leaves were extracted using 70% ethanol. Cytotoxic assay of the FEE in different concentrations (3.13 to 100 µg/ml) against EA.hy926 was done using MTS assay. The FEE’s anti-inflammatory activity was measured with ELISA for the TNF-α and IL-10 parameters. The FEE did not cause cytotoxicity to the EA.hy926 cells, with viability above 80% at the highest concentration. The FEE could decrease the pro-inflammatory TNF-α level and increase the IL-10 level as anti-inflammatory markers in the preeclampsia model at significance level p < 0.05. This study suggests that FEE is a safe alternative herbal medicine for preeclampsia treatment.