Open AccessSolving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy
Author(s) -
Magdalena Donczew,
Katarzyna Ginda,
Jolanta ZakrzewskaCzerwińska,
Dagmara Jakimowicz
Publication year - 2011
Publication title -
postępy higieny i medycyny doświadczalnej
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.275
H-Index - 34
eISSN - 1732-2693
pISSN - 0032-5449
DOI - 10.5604/17322693.933713
Subject(s) - subcellular localization , fluorescence microscope , fluorescence , protein subcellular localization prediction , cytoskeleton , biology , microbiology and biotechnology , fluorescent protein , cell , bacterial cell structure , live cell imaging , bacterial protein , gene , computational biology , green fluorescent protein , bacteria , biochemistry , genetics , cytoplasm , physics , quantum mechanics
We have reviewed how the development of fluorescent markers, triggered by the discovery of green fluorescence protein and its other color variants leading to the establishment of methods for studies of protein interactions with application of fluorescent proteins, affected the view of bacterial cell organization. Application of the new microscopic methods allowed localization of proteins and chromosomal regions, and observation of their migration in real time. These studies revealed the spatial organization of bacterial cells which includes specific subcellular localization of proteins, the presence of dynamic cytoskeletal structures, orchestrated and active segregation of chromosomes, and spatiotemporal gene regulation.