Open AccessMetody badania autofagii oparte na przemianach białek MAP1LC3 i p62/SQSTM1
Author(s) -
Edyta Wysokińska,
Wojciech Kałas
Publication year - 2013
Publication title -
postępy higieny i medycyny doświadczalnej
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.275
H-Index - 34
eISSN - 1732-2693
pISSN - 0032-5449
DOI - 10.5604/17322693.1050029
Subject(s) - autophagy , nutrient deficiency , organelle , biology , blot , microbiology and biotechnology , lysosome , nutrient , biochemistry , ecology , gene , apoptosis , enzyme
Autophagy is a cellular process fundamental for the survival of nutrient deficiency periods and for organelle turnover. Recently much attention has been focused on autophagy as its impairment has been found in many human diseases. Unfortunately, our apparatus for study of the autophagy process is still unsatisfactory and not very well known. In this paper we would like to shed light on and discuss autophagy methods. We present the methods (fluorescence and Western blotting) based on conversions of MAP1LC3 and p62/SQSTM1 proteins, which are the most common markers of the autophagy process.