Open AccessPancreatic β-cell replacement: advances in protocols used for differentiation of pancreatic progenitors to β-like cells
Author(s) -
Muhammad Waseem Ghani,
Ye Li,
Zhao Yun-feng,
Hammad Ghani,
Muhammad Waseem Birmani,
Aamir Nawab,
Lang Guan Cun,
Bin Liu,
Mei Xiao
Publication year - 2015
Publication title -
folia histochemica et cytobiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.384
H-Index - 40
eISSN - 1897-5631
pISSN - 0239-8508
DOI - 10.5603/fhc.a2019.0013
Subject(s) - progenitor cell , stem cell , cellular differentiation , microbiology and biotechnology , biology , pancreas , endocrinology , genetics , gene
Insulin-producing cells derived from in vitro differentiation of stem cells and non-stem cells by using different factors can spare the need for genetic manipulation and provide a cure for diabetes. In this context, pancreatic progenitors differentiating to β-like cells garner increasing attention as β-cell replacement source. This kind of cell therapy has the potential to cure diabetes, but is still on its way of being clinically useful. The primary restriction for in vitro production of mature and functional β-cells is developing a physiologically relevant in vitro culture system which can mimic in vivo pathways of islet development. In order to achieve this target, different approaches have been attempted for the differentiation of pancreatic stem/progenitor cells to β-like cells. Here, we will review some of the state-of-the-art protocols for the differentiation of pancreatic progenitors and differentiated pancreatic cells into β-like cells with a focus on pancreatic duct cells.