Open AccessL1-ORF1p and Ago2 are involved in a siRNA-mediated regulation for promoter activity of L1-5’UTR
Author(s) -
Liu Shi,
Huanhuan Shi,
Lou Liang-liang,
Xianglin Yuan,
Yaping Zhu
Publication year - 2019
Publication title -
folia histochemica et cytobiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.384
H-Index - 40
eISSN - 1897-5631
pISSN - 0239-8508
DOI - 10.5603/fhc.a2019.0006
Subject(s) - promoter , rna silencing , untranslated region , biology , argonaute , three prime untranslated region , rna , microbiology and biotechnology , five prime untranslated region , genetics , rna interference , gene , gene expression
Long interspersed nuclear elements-1 (L1), as the only one self-active retrotransposon of the mobile element, was found to be generally activated in tumor cells. The 5'UTR of L1 (L1-5'UTR) contains both sense and antisense bidirectional promoters, transcription products of which can generate double-strand RNA (dsRNA). In addition, L1-ORF1p, a dsRNA binding protein encoded by L1, is considered to engage in some RNA-protein (RNP) formation. Ago2, one of the RISC components, can bind to dsRNA to form RISC complex, but its role in L1 regulation still remains unclear. Due that the 5'UTR of L1 (L1-5'UTR) contains both sense and antisense bidirectional promoters, so the activities in both string were identified. A dsRNA-mediated regulation of L1-5'UTR, with the feedback regulation of L1-ORF1p as well as other key molecules engaged (Ago1-4) in this process, was also investigated.