Open AccessMicropropagation of Plantago maritima L. - a vanishing species in Poland
Author(s) -
Joanna Makowczyńska,
Emilia Andrzejewska-Golec
Publication year - 2011
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.2009.002
Subject(s) - kinetin , zeatin , explant culture , micropropagation , biology , botany , shoot , hypocotyl , seedling , plantago ovata , plantaginaceae , plantago , callus , cytokinin , in vitro , auxin , biochemistry , gene
A vanishing species in Poland - Plantago maritima L. was regenerated in vitro from tips of shoots (obtained in vitro) and from different explants of 4-week-old seedlings: seedling tips, hypocotyls, cotyledons, roots. Murashige and Skoog basal medium, supplemented with 0.6 pM indole-3-acetic acid in combination with cytokinins 6-benzyladenine, zeatin or kinetin, was used. The plants obtained in the result of micropropagation were normal in appearence. It was proved that Plantago maritima species was amenable to propagation from different kinds of explants. The method may be of significance for protection of sea plantain