Open AccessEffects of various elicitors on the accumulation and secretion of spiroketal enol ether diacetylenes in feverfew hairy root culture
Author(s) -
Anna Stojakowska,
J. Burczyk,
Wanda Kisiel,
Maria Zych,
Antoni Banaś,
Teresa Duda
Publication year - 2011
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.2008.002
Subject(s) - enol ether , chemistry , enol , epoxide , stereochemistry , ether , biochemistry , organic chemistry , catalysis
In a hairy root culture of Tanacetum parthenium treated with yeast extract (YE), silver nitrate (AgNO3) and microalgal glycoproteins (MGPS), contents of four spiroketal enol ether type diacetylenes were mesured. The elicitors transiently reduced contents of three constitutive spiroketal enol ethers and selectively enhanced accumulation of cis-C13-spiroketal enol ether epoxide ((E)-3,4-epoxy-2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4.4]nonane) in the roots. The most abundant formation of cis-C13-spiroketal enol ether epoxide was observed after 48-96 h of AgNO3 treatment and 96 h of YE treatment (over 3-fold increase compared with the control). The applied elicitors caused enhanced liberation of cis-C13-spiroketal enol ether epoxide to the culture medium. The results show that diacetylene accumulation pattern in the elicited hairy roots is affected in a similar manner, irrespectively of the elicitor applied