Open AccessLocalization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum)
Author(s) -
Marzena Sujkowska,
Wojciech Borucki,
W. Golinowski
Publication year - 2011
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.2006.006
Subject(s) - pisum , acid phosphatase , bacteria , sativum , apoplast , biochemistry , biology , enzyme , alkaline phosphatase , enzyme assay , root nodule , microbiology and biotechnology , symbiosis , botany , cell wall , genetics
Changes in the activity of acid phosphatase (AcPase) in the apoplast of pea root nodule were investigated. The activity was determined using lead and cerium methods. The results indicated a following sequence of AcPase activity appearance during the development of the infection thread: 1) low AcPase activity appears in the outer part of cells of symbiotic bacteria; 2) bacteria show increased AcPase activity, and the enzyme activity appears in the thread walls; 3) activity exhibits also matrix of the infection thread; 4) bacteria just before their release from the infection threads show high AcPase activity; 5) AcPase activity ceases after bacteria transformation into bacteroids. The increase in bacterial AcPase activity may reflect a higher demand for inorganic phosphorus necessary for propagation of the bacteria within the infection threads and/or involved in bacteria release from the infection threads