Open AccessPhylogenetic studies of liverworts from the genus Pellia using a new type of a molecular marker
Author(s) -
Andrzej Pacak,
Paweł Fiedorow,
Zofia Szweykowska-Kulińska
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.2002.027
Subject(s) - biology , phylogenetic tree , primer (cosmetics) , genus , rapd , botany , phylogenetics , genetics , gene , genetic diversity , chemistry , population , demography , organic chemistry , sociology
In order to analyse taxonomically liverworts from the genus Pellia a new type of molecular marker was applied using two kinds of primers. The first one is a typical RAPD primer whereas the second one is a universal primer whose nucleotide sequence is based on a consensus sequence common to all tRNA molecules. Using these markers phenetic and phylogenetic trees were constructed for all the species of the genus Pellia, using the liver-wort species Aneura pinguisas the outgroup. Additionally, it was demonstrated that the allopolyploidal species P. borealis, which originated as a result of the hybridization of two parent species: P. epiphylla-species S and P. epiphylla-species N, is more closely related to P. epiphylla-species N. This is because both: mitochondria and chloroplasts in P. borealis are from P. epiphylla-species N