Open AccessIdentification of Lycopersicon spp. hybrids on the basis of morphological and molecular (RAPD) properties as well as evaluation of resistance to Tomato Spotted Wilt Virus (TSWV)
Author(s) -
Zbigniew Rusinowski,
M. Śmiech,
M. Kamińska,
K. Niemirowicz-Szczytt
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.2002.011
Subject(s) - hybrid , lycopersicon , biology , rapd , horticulture , plant stem , botany , habit , psychology , population , demography , sociology , genetic diversity , psychotherapist
The aim of the study was to evaluate the plants obtained as a result of cultivated tomato crosses with wild species meant to transfer resistance to TSWV. Six viable plants were obtained from L. esculentum x L. chilense and L. esculentum x L. peruvianum crosses after the application of in vitro embryo culture. In terms of such morphological traits as growth habit of plants, size and shape of leaves, the length and colour of internodes in branching stems, the plants displayed intermediate traits, resembling, nonetheless, the wild form. RAPD analysis with 8 primers revealed that all the hybrids had bands typical of the paternal forms. This confirms the paternal component in hybrid development. As far as the resistance to Polish TSWV isolates is concerned, two hybrids exhibited a high level of resistance, similar to negative control, three hybrids - enhanced resistance and one hybrid was susceptible to TSWV infection