Open AccessPreliminary results of indole alkaloids production in different roots of Catharanthus roseus cultured in vitro
Author(s) -
Agnieszka Pietrosiuk,
M. Furmanowa
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.2001.033
Subject(s) - catharanthus roseus , agrobacterium , biology , botany , in vitro , vindoline , indole test , vinblastine , transformation (genetics) , gene , biochemistry , genetics , chemotherapy
Six groups of untransformed and hairy root cultures of Catharunthus roseus (L.) G. Don were established. Agrobacterium rhizogenes strains: ATCC 15834, LBA 9403, and TR 105 were used for infection of the 3-week old rooted plantlets of C. roseus. The highest contents of examined indole alkaloids were found in: roots of intact plants - yohimbine and serpentine; in hairy roots - catharanthine. Vinblastine and ajmalicine were detected in untransformed roots of plants regenerated in vitro, and transferred to the soil for 5 months