Preliminary results of indole alkaloids production in different roots of Catharanthus roseus cultured in vitro | Zendy

Agnieszka Pietrosiuk | Zendy; Mirosława Furmanowa | Zendy

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Preliminary results of indole alkaloids production in different roots of Catharanthus roseus cultured in vitro

Author(s) -

Agnieszka Pietrosiuk,

Mirosława Furmanowa

Publication year - 2013

Publication title -

acta societatis botanicorum poloniae

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.297

H-Index - 29

eISSN - 2083-9480

pISSN - 0001-6977

DOI - 10.5586/asbp.2001.033

Subject(s) - catharanthus roseus , agrobacterium , biology , botany , in vitro , vindoline , indole test , vinblastine , transformation (genetics) , gene , biochemistry , genetics , chemotherapy

Six groups of untransformed and hairy root cultures of Catharunthus roseus (L.) G. Don were established. Agrobacterium rhizogenes strains: ATCC 15834, LBA 9403, and TR 105 were used for infection of the 3-week old rooted plantlets of C. roseus. The highest contents of examined indole alkaloids were found in: roots of intact plants - yohimbine and serpentine; in hairy roots - catharanthine. Vinblastine and ajmalicine were detected in untransformed roots of plants regenerated in vitro, and transferred to the soil for 5 months

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