Open AccessMolecular cytogenetic studies in Chenopodium quinoa and Amaranthus caudatus
Author(s) -
Jolanta Małuszyńska,
Luz Gómez Pando,
Bożena Kolano
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.2001.011
Subject(s) - biology , chenopodium quinoa , fluorescence in situ hybridization , chromosome , staining , microbiology and biotechnology , genetics , amaranth , botany , gene , biochemistry
Chenopodium quinoa Wild. and Amaranthus caudatus L., two plant species from South America, have small and numerous chromosomes. Looking for chromosome markers to distinguish pairs of homologous chromosomes double fluorescence staining, in situ hybridization with 45S rDNA and silver staining were applied. Fluorescent in situ hybridization with 45S rDNA has shown two sites of hybridization occurring on one pair of chromosomes in qunion genre (lines PQ-1, PQ-8). The number of RDA loci in Amaranth's caudate L. genre depends on the accession. Kiwicha 3 line has one pair of chromosomes with signals and Kiwicha Molinera cultivar two pairs. All observed rDNA loci were active. After chromomycin/DAPI staining in all cases, except Kiwicha Molinera cultivar, the CMA3 positive bands co-localized with signals of in situ hybridization with rDNA. In Kiwicha Molinera the number of CMA+ bands was higher than the number of 45S rDNA signals after FISH