Effect of pectinase on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.
Author(s) -
Janusz Malarz,
Wanda Kisiel
Publication year - 2013
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.2000.014
Subject(s) - hairy root culture , sesquiterpene , pectinase , elicitor , agrobacterium , lactuca , botany , asteraceae , horticulture , biology , enzyme , transformation (genetics) , biochemistry , gene
Direct addition of pectinase as an elicitor to the hairy root culture of Lactuca virosa L. transformed with Agrobacterium rhizogenes stimulates the accumulation of constitutive sesquiterpene lactones - lactuside A and crepidiaside B. The highest amounts of lactuside A (80% above the control level) and crepidiaside B (39% above the control level) have been found 24 and 48 hours, respectively, after pectinase treatment
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