Open AccessMicropropagation of phytoplasma-affected Limonium sinuatum Mill. plants
Author(s) -
E. Gabryszewska,
M. Kamińska,
M. Korbin,
Anna Rudzińska-Langwald
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.2000.013
Subject(s) - chlorosis , shoot , biology , phytoplasma , axillary bud , micropropagation , plant stem , explant culture , botany , scrophulariaceae , horticulture , in vitro , polymerase chain reaction , biochemistry , restriction fragment length polymorphism , gene
Healthy and AY-affected plants of L.sinuatum have been propagated in vitro for 12 months on the media with and without cytokinins. In the contrary to the healthy plants the phytoplasma affected statice showed abnormal proliferation of the axillary shoots, shortening of the internodes, smaller leaves and severe chlorosis. On the medium without cytokinins, diseased plants proliferated and formed 7.0 axillary shoots per explant but the healthy ones only formed 2.3 shoots; however, the fresh weight of them was similar. On the media with cytokinins, the multiplication rate and fresh weight of healthy shoots greatly in-creased, but of the diseased plants were on the same laevel or decreased. During tissue culture phytoplasma could be detected in symptomatic plants by PCR as well as electron microscope however, the phytoplasmas showed the symptoms of degeneration