Heterochromatin organization in metaphase chromosomes and interphase nuclei of Dasypyrum breviaristatum (Lindb) Frederiksen | Zendy

Domenico Pig | Zendy; Incoronata Galasso | Zendy; Antonio Blanco | Zendy; Roberto Cremonini | Zendy

Open Access

Heterochromatin organization in metaphase chromosomes and interphase nuclei of Dasypyrum breviaristatum (Lindb) Frederiksen

Author(s) -

Domenico Pig,

Incoronata Galasso,

Antonio Blanco,

Roberto Cremonini

Publication year - 2013

Publication title -

acta societatis botanicorum poloniae

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.297

H-Index - 29

eISSN - 2083-9480

pISSN - 0001-6977

DOI - 10.5586/asbp.2000.001

Subject(s) - heterochromatin , biology , metaphase , ploidy , interphase , karyotype , genetics , dna , chromosome , chromatin , gene

Metaphase chromosomes of Dasypyrum breviaristatum (Lindb f) Frederiksen, a tetraploid wild species, were differentially stained with C-banding and fluorochromes in order to aquire information on heterochromatin chromosomal distribution and composition. DNA content and relative amount of nuclear heterochromatin were determined by cytophotometric analysis after Feulgen reaction. The results were compared to those of Dasypyrum villosum (L.) P. Candargy, a diploid species of the same genus. The achieved information indicate that D. breviaristatum and D. villosum differ in the composition, organization and distribution of heterochromatin, and may suggest that the telomeric regions of the chromosomes of the two species are more differentiated than the centromeric ones, as a result of a long lasting divergence between the two species

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