Open AccessEffect of methyl jasmonate on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.
Author(s) -
Janusz Malarz,
Wanda Kisiel
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1999.017
Subject(s) - methyl jasmonate , sesquiterpene , hairy root culture , lactuca , agrobacterium , sesquiterpene lactone , botany , chemistry , horticulture , traditional medicine , biology , biochemistry , medicine , transformation (genetics) , gene
Hairy roots of Lactuca virosa L. transformed with Agrobacterium rhizogenes and treated with methyl jasmonate, are capable of producing sesquiterpene lactones - crepidiaside B and lactuside A in amounts higher than in untreated roots. An HPLC analysis showed the highest content of crepidiaside B after 24 hours of methyl jasmonate treatment (60% above the control). The increase in the content of lactuside A was relatively small and reached 27% after 48 hours of the treatment, in comparison with the control