Open AccessRegeneration of Paeonia mlokosewitschii Lom. and P. tenuifolia L. in vitro from different explants
Author(s) -
T. Orlikowska,
Agnieszka Marasek,
D. Kucharska
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1998.026
Subject(s) - explant culture , callus , shoot , biology , regeneration (biology) , botany , petal , in vitro , murashige and skoog medium , basal shoot , microbiology and biotechnology , biochemistry
The pattern of regeneration from tissues of Paeonia mlokosewitschii and P. tenuifolia cultured in vitro in the same chemical conditions depended on the initial explant. Direct shoot regeneration was obtained from the bases of petioles and petals, and leaf veins. Vegetative initial buds and regenerated in vitro shoots produced on their bases slowly growing nodular callus which was very productive in repetitive shoot regeneration. The tops of stems, flower bases, sepals, petals and ovary walls produced small callus which regenerated white and red spherical structures within 1.5 years. After that time also from those cultures arised nodular, shoot regenerating callus developed