Open AccessRhodiola rosea in vitro culture - phytochemical analysis and antioxidant action
Author(s) -
M. Furmanowa,
Ewa Skopińska-Różewska,
E Rogala,
Małgorzata Hartwich
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1998.009
Subject(s) - rhodiola rosea , salidroside , phytochemical , caffeic acid , callus , antioxidant , rhodiola , in vitro , botany , chemistry , traditional medicine , shoot , biology , biochemistry , chromatography , medicine
Various Rhodiola rosea organs and tissues from in vitro culture were studied in two areas: searching for the biological active four phenolic compounds and measurement of antioxidant activity of dry residues of EtOH-extracts soluble in PBS using chemiluminescence method. For phytochemical investigation HPLC method was used. Salidroside was typical for organs of intact plant, rosavin for roots of different origin and shoots of intact plants, triandrin was more typical for tissue cultured in vitro, but it was found in all studied samples. Caffeic acid was detected mainly in green and yellow lines of callus, less in other Rhodiola rosea plant material. The highest antioxidant activity showed the extract from 1,5-years-old callus of green and yellow line, which contained caffeic acid and triandrin. Further observations are in progress