Open AccessRegeneration of Pharbitis nil from immature embryos by somatic embryogenesis
Author(s) -
Alina Trejgell,
Andrzej Tretyn
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1997.020
Subject(s) - somatic embryogenesis , hypocotyl , pharbitis nil , embryo , biology , botany , agar , gibberellic acid , horticulture , embryogenesis , germination , microbiology and biotechnology , bacteria , genetics
Immature embryos of Pharbitis nil Chois. were used for the study. They were isolated from previously sterilized fruits and afterwards transferred to Murashige and Skoog (MS) growth medium with 0.8% agar. Immature embryos were cut across their axis. After 6-8 weeks of cultivation in the injury place (hypocotyl-root region) somatic embryos were formed. These embryos were isolated and each of them was transferred into a separate tube containing MS supplemented with naphthaleneacetic acid (NAA; in concentration 0.1 mg•dm-3 ) and gibberellic acid (GA3; in concentration 0.5 mg•dm-3). Under these conditions about 26% of somatic embryos regenerated into complete plants. Two-three weeks after the photoperiodic induction flowers appeared on these plants. A few weeks after pollination normal seeds were developed from these flowers