Open AccessSomatic embryogenesis in Lolium multiflorum suspension culture
Author(s) -
Margarita Pavlova,
Е.L. Kordyum
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1996.007
Subject(s) - picloram , lolium multiflorum , somatic embryogenesis , dicamba , callus , suspension culture , biology , botany , somatic cell , embryo , lolium , embryogenesis , meristem , shoot , cell culture , microbiology and biotechnology , agronomy , poaceae , weed control , biochemistry , genetics , gene
The embryogenic cell suspension was obtained from immature embryos of Lolium multiflorum through a callus culture. Somatic embryogenesis was induced by addition of 2,4-D, dicamba and picloram in 0,5 mg/l concentrations in MS liquid nutrient medium. It was shown that somatic embryos arised from single cells. In globular embryoids, the meristematic cells are characterized by the presence of phytoferritin inclusions in the leucoplasts