Open AccessAnalysis of poly(A) + RNA distribution during maize somatic embryogenesis using digoxigenated oligo-dT probes
Author(s) -
Raageeva Bimal,
F.B.F. Bronsema,
M.T.M. Willemse,
J.H.N. Schel
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1996.004
Subject(s) - callus , somatic embryogenesis , suspensor , embryo , biology , somatic cell , embryogenesis , microbiology and biotechnology , botany , genetics , gene
The pattern of total transcription activity in terms of steady state levels of poly(A)+ containing mRNA during callus initiation and somatic embryogenesis in a high (A188) and a low (A632) embryogenic line of maize was analyzed using digoxigenin labelled oligo-dT probes. A gradual increase and a preferential accumulation of label was observed in both lines, differing temporally up to 4 days in culture. In the A188 line of maize the callus gave rise to somatic embryos. The globular embryos showed less label than the callus; this labelling was mostly present in the basal part of the embryos. At a later stage upper embryogenic and lower non-embryogenic layers were observed in the A188 callus, showing conspicuous differences in the amount of label. In the late globular stage the poly(A)+ RNA signals were seen all over the embryo but at the junction of the suspensor and the callus tissue no label was observed