Open AccessProgress towards sugar beet improvement through somatic hybridization I. Inactivation of nuclei and cytoplasm in donor and recipient protoplasts
Author(s) -
E. Jażdżewska,
A. Niklas,
A. Majewska-Sawka
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1995.044
Subject(s) - protoplast , sugar beet , somatic cell , biology , somatic fusion , iodoacetic acid , plating efficiency , cytoplasm , botany , staining , agarose , biochemistry , gene , horticulture , genetics , enzyme , cell
The isolation and culture of suspension-derived protoplasts from two sugar beet (Beta vulgaris L.) genotypes are described. Immobilization of protoplasts in agarose resulted in high frequency divisions and microcallus regeneration, with plating efficiency (PE) being clearly genotype-dependent. In further studies towards asymmetric fusion experiments, the effect of different doses of ultraviolet radiation (UV) and iodoacetic acid (IA) on protoplast physiology was assessed. Viability of both treated (UV, IA) and untreated protoplasts (control) was determined by FDA staining, and the biological effect was evaluated by testing the ability of protoplasts to divide and to form calli. The results are discussed in terms of the applicability of the methods for the production of asymmetric protoplasts suitable for somatic hybridization within the genus Beta