Open AccessHairy root culture of Lactuca virosa L.
Author(s) -
Anna Stojakowska,
Janusz Malarz,
Wanda Kisiel,
S Kohlmünzer
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1995.006
Subject(s) - hairy root culture , asteraceae , sucrose , explant culture , sesquiterpene , lactuca , agrobacterium , botany , biology , horticulture , biomass (ecology) , chemistry , food science , in vitro , transformation (genetics) , agronomy , biochemistry , gene
A hairy root culture of Lactuca virosa L. (Asteraceae) was initiated by infection of the leaf explants of the aseptic plants with Agrobacterium rhizogenes - strain LBA 9402. One of the obtained clones which showed the most favourable growth rate was used for further investigation. An influence of different nutrient media and sucrose concentrations on the biomass accumulation was studied. The most advantageous for the biomass increment were media: MS and MS containing macronutrients reduced to half concentration, supplemented with 5% sucrose. A preliminary chromatographic analysis of the tissue extract revealed the presence of sesquiterpene lactones characteristic of the roots of the intact plant