Open AccessThe intracellular localization of malate dehydrogenase isoenzymes in Pisum arvense roots
Author(s) -
Genowefa Kubik-Dorosz
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1986.051
Subject(s) - pisum , malate dehydrogenase , biochemistry , biology , plastid , nad+ kinase , dehydrogenase , isoelectric focusing , mitochondrion , enzyme , chloroplast , gene
Mitochondria and plastids were isolated from Pisum arvense root cells by sucrose density gradient centrifugation. The individual subcellular fractions so obtained were subjected to isoelectric focusing on cellulose acetate strips. Mitochondria and plastids each contained one NAD -malate dehydrogenase, while three isoenzymes were associated with the supernatant