Open AccessThe localization of nitrite reductase, glutamate synthase and malate metabolism enzymes in Pisum arvense L. roots
Author(s) -
Genowefa Kubik-Dobosz,
Grażyna Kłobus
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1985.008
Subject(s) - biochemistry , glutamate synthase , malate dehydrogenase , pisum , biology , nitrite reductase , glutamate dehydrogenase , reductase , malic enzyme , microsome , plastid , mitochondrion , atp synthase , cytosol , enzyme , nitrate reductase , dehydrogenase , glutamate receptor , chloroplast , receptor , gene
Centrifugation of a homogenate made from Pisum arvense L. roots in a sucrose density gradient enabled the separation of the plastid fraction from mitochondria and microsomes. The presence of nitrite reductase and glutamate synthase was demonstrated in the plastids. Malic enzyme activity was not linked with any organelle fraction and was found only in the cytosol. High malate dehydrogenase activity was found in the mitochondria fraction, although its activity was also determined in plastids. The results suggest that malic acid metabolism in plastids may be the source of reduced pyridine nucleotides for reactions catalysed by nitrite reductase and glutamate synthase