Open AccessStudies on Medicago lupulina saponins. 2. Isolation, chemical characterization and biological activity of saponins from M. lupulina tops
Author(s) -
Piotr Górski,
Marian Jurzysta,
Stanisław Burda,
Wiesław Oleszek,
Michał Płoszyński
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1984.048
Subject(s) - rhamnose , hydrolysate , chemistry , arabinose , aglycone , xylose , saponin , glycoside , glucuronic acid , fraction (chemistry) , galactose , triterpene , chromatography , biochemistry , fermentation , polysaccharide , stereochemistry , hydrolysis , medicine , alternative medicine , pathology
Two pure saponin fractions from the tops of M. lupulina were isolated and characterized. The fractions named Ma and Ss varied in composition and biological activity. It was shown by TLC that fraction Ma contained 5 components. In the acid hydrolysates of that fraction only one aglycone (medicagenic acid) and 4 sugars: rhamnose, xylose, arabinose, glucuronic acid were found. The Ss fraction contained 11 components. In its acid hydrolysates, soyasapogenols B, C, D, E, F and two new aglycones N and An were found. The same sugars as above and additionally glucose and galactose were identified. It was found that the Ma fraction of saponins (medicagenic acid glycosides) was highly fungistatic, haemolytic and toxic to fishes. The Ss fraction of saponins (soyasapogenol glycosides) showed no such activity