Open AccessAcid phosphatase from stored Poa pratensis caryopses and its ability for binding to lectins
Author(s) -
Irena LorencKubis,
Bronisława Morawiecka
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1984.019
Subject(s) - acid phosphatase , phosphatase , caryopsis , enzyme , biochemistry , enzyme assay , biology , chemistry , botany , poaceae
The effect of the storage period of Poa pratensis caryopses on acid phosphatase activity and on the ability of this enzyme to interact with lectins has been studied. It has been shown that after ten years of caryopses storage, the activity of acid phosphatase decreased about 50 per cent, while the content of proteins and carbohydrates did not change. The decrease of enzyme activity during the long period of storage was found only in seeds, but not in chaffs. Acid phosphatase was isolated from caryopses stored one, two, three, five and ten years. The enzyme showed the ability to bind to immoblized as well as to free conA during the whole period of storage, hut did not react with Wheat Germen Agglutinin (WGA). The activation of acid phosphatase by binding to conA decreased with the length of storage period