Open AccessEffect of ammonium nutrition on the nitrate utilization, nitrate reductase actvity and growth of Spirodela polyrrhiza
Author(s) -
Ewa Tatkowska,
J. Buczek
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1983.027
Subject(s) - nitrate reductase , nitrate , ammonium , chemistry , nitrogen , nitrogen assimilation , assimilation (phonology) , nuclear chemistry , inorganic chemistry , philosophy , linguistics , organic chemistry
The influence of NH4+ ions on nitrate assimilation and growth of sterile Spirodela polyrrhiza cultures was investigated. S. polyrrhiza utilises both the nitrate and the ammonium form of nitrogen, it prefers, however, NH4+. Ammonium ions present in the nitrate medium inhibit the activity of nitrate reductase (NR), but they do not affect enzyme 'induction and only slightly reduce N03- uptake. These results sugest that the inhibitory effect of NH4+ on the NR activity is the main cause of the decrease in N03- assimilation by S. polyrrhiza cultures growing in nitrate-ammonium medium