Open AccessInvestigations on the ultrastructure of the callus tissue of Liriodendron tulipifera L. developed in vitro
Author(s) -
Barbara Stefaniak,
Adam Woźny
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1983.001
Subject(s) - callus , ultrastructure , plant stem , organogenesis , biology , botany , in vitro , tissue culture , biochemistry , gene
Fragments of internodes, petioles and fragments of leaves of Liriodendron tulipifera L. were cultured in vitro in order to induce them to regeneration. Abundant callus tissue was only produced by unlignified internodes and petioles. Application of various combinations of media was not successful in inducing callus organogenesis. The callus did not survive long and cytological analysis of its cells revealed the presence of severe abnormalities